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Image Search Results
Journal:
Article Title: When Pigs Fly: Immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis
doi: 10.1016/j.mrgentox.2011.01.009
Figure Lengend Snippet: Three bivariate graphs illustrate the gating logic used for the mutant scoring application described herein. In order for events to be considered an RBC and subject to evaluation of mutant phenotype versus wild-type phenotype status, they need to exhibit light scatter characteristics of cells (panel A), low/moderate SYTO 13-associated fluorescence consistent with erythrocytes and reticulocytes but not leukocytes (panel B), and low APC-associated fluorescence consistent with cells but not Counting Beads (panel C). Thus, while the Lympholyte reagent physically removes the bulk of platelets and leukocytes, and while Counting Beads are present in both Pre- and Post-Column specimens, this gating strategy ensures that mutant phenotype RET and RBC frequencies are not affected by these events.
Article Snippet: Phosphate buffered saline (PBS; phenol red and divalent cation free) was from Mediatech, Inc., Herndon, VA.
Techniques: Mutagenesis, Fluorescence